Repair of nicks Nick (DNA)

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minimalistic mechanism of dna nick sealing dna ligase


in order ligate these fragments together, ligase progresses through 3 steps:



one particular example of ligase catalyzing nick closure e. coli nad+ dependent dna ligase. liga relevant example structurally similar clade of enzymes found across types of bacteria. liga drives process of ligation wrapping protein clamp around dna , creating large protein conformational changes , remodeling of active site.


ligases have metal binding site capable of recognizing nicks in dna. ligase forms dna-adenylate complex, assisting recognition. human dna ligase, forms crystallized complex. complex, has dna-adenlyate intermediate, allows dna ligase institute conformational change in dna isolation , subsequent repair of dna nick.








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