Protein FAM149B1

1 protein

1.1 general properties
1.2 splice variants
1.3 structure
1.4 subcellular localization
1.5 post-translational modifications

1.5.1 acetylation
1.5.2 phosphorylation
1.5.3 sumoylation

protein
general properties

the primary protein encoded fam149b1 gene 583 amino acids in length , has molecular weight of 64 kdal. protein contains conserved protein domain, duf3719 located @ amino acids 115-179. isoelectric point of protein before post-translational modifications 6.3, , isoelectric point relatively conserved in protein s isoforms, in similar composition of exons. protein considered serine rich, in expresses higher serine composition relative composition of other human proteins. high serine composition seen in gene s orthologues.

splice variants

the splice variants of protein demonstrate shared qualities of protein translated primary transcript. because each isoform different length , contains various combinations of available exons, there variances in isoelectric point , molecular weight. isoforms closest weight , exon composition primary transcript share these characteristics. protein isoforms missing conserved duf3719 domain isoforms x5 , x6 because domain contained between exons 3-6.

structure

there negative charge cluster amino acids 212 239. negative charge clusters coordinate calcium, or magnesium or zinc ions, mannose-binding protein, or aminopeptidase. protein contains no positive or mixed charge clusters. secondary structure of protein predicted combination of alpha-helices few predicted beta-sheet structures.

this figure shows predicted 3d structure of human fam149b1 protein. secondary structures contributing tertiary structure alpha-helices , 1 predicted beta-sheet turn.



subcellular localization

immunofluorescent stain shows expression of fam149b1 highly expressed in nucleus.

the subcellular location of protein nucleus. there leucine zipper pattern in protein beginning @ amino acid 347.

post-translational modifications
acetylation

the third amino acid in protein sequence, serine, predicted acetylated.

phosphorylation

there multiple predicted phosphorylation sites on various serine, tyrosine, , threonine amino acids predicted protein sequence. conserved duf3719 domain contains 7 predicted phosphorylation sites.

sumoylation

one predicted sumoylation site identified in protein sequence @ k267.

a schematic diagram of fam149b1 protein after post translational modifications. duf3719 domain displayed.